Synchronization of Saccharomyces cerevisiae can be achieved in different ways. First, block-and-release methods, where cells are synchronized in a specific cell cycle phase and released afterwards, can be used. Most prominent is the synchronization in G1 phase with alpha factor. Second, we use elutriation. For this synchronization method, cells are loaded in a specific centrifugation chamber. Using a buffer flow in the opposite direction of centrifugal force cells can be sorted depending on their size (surface/volume ratio). By increasing the flow we can washout and then harvest the smallest, new born G1 cells.